Genomic DNA is often cloned into plasmid vectors using DNA adapters to add the required restriction sites to a gene of interest, prior to ligation. Direct sequencing of the recombinant plasmid is often performed to verify the sequence integrity of the cloned fragment. The source of the insert for cloning may be genomic DNA, a cDNA, a portion of another plasmid, or a linear DNA fragment. HIRAGENE offers you to design your cloning project.